Based on ultrasonic cavitation effect. The ultrasonic probe transmits high-frequency vibrations to the cell suspension, causing the liquid to produce areas of high pressure and low pressure. The bubble in the low pressure region expands, and the bubble in the high pressure region implodes, releasing shock waves and microjets that impact the cell and cause it to burst. At the same time, ultrasonic drive liquid convection, to ensure that the ultrasonic energy evenly dispersed, improve the crushing efficiency.
Compared with the grinding method, the ultrasonic cell crusher can finish the cell breaking quickly, avoiding the damage of the biological molecules caused by uneven grinding and heat production. Compared with the repeated freeze-thaw method, it can be operated at normal or low temperature, reducing the damage to temperature-sensitive biomolecules, and the operation is simpler.
It is used to extract proteins, enzymes, antibodies and other biopharmaceutical raw materials in microbial or mammalian cells, and extract antigen components in vaccine research and development.
Obtain intracellular nucleic acid for gene cloning, sequencing and other experiments; The broken cells release proteins and carry out proteomic studies.
Selective cell fragmentation to study organelle function and metabolic pathway, preparation of cell lysate to study cell signal transduction, apoptosis and other processes.
Looking forward to the future, with the continuous progress of science and technology, ultrasonic cell crusher will develop in the direction of more intelligent, efficient and refined. Emerging ultrasound technologies and devices will continue to emerge, further improve the effect and accuracy of cell fragmentation, provide more powerful technical support for research in life science, medicine and other fields, and promote the continuous innovation and development of related fields.
