The flavonoids in amomum kernel have various biological activities, but the traditional organic solvent extraction method has some problems, such as high energy consumption, high cost and harm to health. As a new type of green solvent, ultrasound-assisted natural eutectic solvent combined with ultrasonic technology is expected to overcome these shortcomings.
Grind the dried amomum nuts and sieve them for later use. Using betaine and L-proline as hydrogen bond acceptors and many compounds as hydrogen bond donors, 22 kinds of ultrasus-assisted natural eutectic solvents were synthesized and their structures and properties were characterized.
The high efficiency ultrasound-assisted natural eutectic solvent was selected, and the single factor experiment was conducted to investigate the effects of water content, solid-liquid ratio, extraction temperature, ultrasonic power and time on total flavonoid content. The selection of ultrasound-assisted natural eutectic solvents was compared with traditional solvents to determine the content of total flavonoids, observe the microstructure and evaluate the antioxidant capacity.
which consists of betaine and glycerol, was selected as the best solvent. Fourier transform infrared spectroscopy 
and thermogravimetric analysis confirmed that the formed hydrogen bond and had good thermal stability.
Ultrasound-assisted emulsification and ultrasound-assisted natural eutectic solvent method were superior to traditional solvents in terms of total flavonoid content, antioxidant activity and extraction types, and could effectively destroy cell structure and promote extraction.
Ultrasound-assisted natural eutectic solvent has low cytotoxicity. extract showed concentration-dependent anti-inflammatory in cells stimulated by lipopolysaccharide. It has significant anti-proliferation and anti-migration effects on cells, and has little effect on normal cells.
A green and efficient method for extracting flavonoids from amomum kernel with ultrasonic-assisted ultrasonic-assisted natural eutectic solvent was established. is an ideal extraction solvent, and the content of total flavonoids is higher under optimized conditions. The method is more efficient than traditional methods, and the extract has good stability, good biocompatibility with ultrasound-assisted natural eutectic solvent, and the extract has anti-inflammatory and anticancer activities, which can be directly used in food and pharmaceutical fields.
