Vitamin C is essential for health, but ordinary vitamin C is easily broken down in the gastrointestinal tract after oral administration and has low bioavailability.
Using high purity lecithin, soybean lecithin is economical, egg yolk lecithin has high biological activity; Select high purity vitamin C powder; Use ethanol or chloroform to dissolve phospholipids, pay attention to its volatility and flammability; The liposomes are stabilized by controlling pH with phosphate buffers.
Rotary evaporator is used to remove solvent and form phospholipid film. The ultrasonic processor disperses the phospholipid film into liposomes and wraps vitamin C, which can also be replaced by a small ultrasonic cleaning machine. Dialysis bags or ultrafiltration devices to remove unwrapped vitamin C and organic solvent residues; The stirrer ensures that the solution ingredients are evenly mixed.
● Dissolve an appropriate amount of phospholipids in ethanol or chloroform and form a uniform phospholipid solution with the help of a blender.
● Transfer the phospholipid solution to the round-bottom flask of the rotary evaporator, rotary evaporation under reduced pressure, and form a phospholipid film on the inner wall of the flask.
● Add a buffer containing vitamin C powder to the flask containing phospholipid film, stir to hydrate the phospholipid film and form a multi-layer liposome suspension to achieve vitamin C encapsulation.
● The suspension is transferred to an ultrasonic processor where ultrasonic vibrations disperse the multi-layer liposomes into a single layer of liposomes to optimize the coating effect, during which the temperature is controlled by an ice bath to prevent damage to the liposomes and vitamin C.
● Use a dialysis bag or ultrafiltration device to remove unwrapped vitamin C and organic solvent residues and obtain a pure liposome vitamin C solution.
Homemade liposome vitamin C can not only deepen our understanding of nutritional supplements, but also enjoy hands-on fun while obtaining vitamin C products with high absorption efficiency.
